Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 41)

Gene Pulser MXcell™ System Manual | Pre-Set Protocols
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Refer to the page numbers listed in Table 6 (starting on page 32) for more information about
each set of protocols.
Table 6. List of pre-set protocols and their uses.
Protocol name
Number
of wells
When to use this protocol
Protocols for well sets with four or six wells (page 34)
1. Opt mini 96 well/ Sqr.Exp 24 Use to rapidly determine optimal waveform
and conditions.
2. Opt mini 96 well/ Sqr 24 Use to rapidly to determine optimal conditions
for square-wave protocols
3. Opt mini 96 well/ Exp 24 Use to rapidly determine optimal conditions
for exponential protocols.
4. Opt 96 well/ Sqr, NP, D 16 Use after optimal square-wave conditions
have been determined to enhance specifically
cell viability and improve efficiency.
Whole plate protocols (page 36)
5. 96 well/ Exp 96 Use for initial protocol set-up for many cell
types.
6. 2 4well/ Exp 24 Use for initial protocol set-up for many cell
types.
7. 12 well/ Exp 12 Use for initial protocol set-up for many cell
types.
8. 96 well/ Sqr 96 Use for initial protocol set-up for many cell
types.
9. 24 well/ Sqr 24 Use for initial protocol set-up for many cell
types.
10. 12 well/ Sqr 12 Use for initial protocol set-up for many cell
types.
Well set protocols (page 37)
11. 96 well/ Exp,Vgrad/ Cgrad 96 Use when working with new cells lines that
traditionally apply exponential waveforms.
This protocol fine tunes conditions and
includes replicates.
12. 96 well/ Sqr,Vgrad/ Dgrad 96 Use when working with new cells lines or
when square-waveform protocols are normally
applied. This protocol fine tunes conditions
and includes replicates.