Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 40)

Gene Pulser MXcell™ System Manual
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5 Pre-Set Protocols
The Gene Pulser MXcell™ electroporation system provides a large set of pre-set protocols
developed by scientists at Bio-Rad Laboratories. Use these protocols to quickly run an
optimization experiment.
The pre-set protocols have been named using these rules: Each begins with a three to eight
letter description and type of electroporation plate (12, 24, or 96-well), followed by a forward
slash and the type of waveform (
EXP or SQR). The end of the name lists the specific
parameter values with the abbreviated names.
The lists and tables in this chapter use abbreviations for the protocol names and
parameters:
• Voltage in volts (V)
• Current in microfarads (μF)
• Resistance in ohms
(Ω)
• Duration in milliseconds (ms)
• Number of pulse is the number of individual pulses (NP)
• Pulse interval is the time between each pulse (s)
• Grad is a gradient
• Exp is exponential waveform
• Sqr is square waveform