Bio-Rad Gene Pulser MXcell™ Electroporation System User Manual

Page 30

Protocol Set-Up Option

Press the arrow keys to select a parameter, then press the alphanumeric keys to enter
a new value. Press

ENTER to confirm the entry.

TIP: Press

Clear to delete the value.

This example shows some values for each parameter in the pulse with a square
waveform (

SQR):

NOTE: The

P in the lower right corner indicates that the required parameters are

complete, and the

PULSE button is ready.

TIP: You can also edit a well set by selecting

Edit well Set? using the arrow

keys to move the cursor. Press ENTER to begin editing. For more information
about well set programming, see Step 5 "Select a waveform (

EXP or SQR)." on

page 22.

6. (Optional) Press

Save to save the changes in the protocol (page 28).

7. Press the

PULSE button to electroporate the sample.

TIP: Once you start a protocol, you can stop the experiment if needed. To stop a
protocol, press and hold the

PULSE button. When the protocol stops, the screen

displays the last pulse (see “Last Pulse Option” on page 26).

ELL

ROGRAMMING

To program a plate with well sets that run different protocols, follow these instructions:

1. Select

Protocol Set-up in the home screen.

Press

ENTER to confirm the selection.

2. Select a plate size.

Press the arrow keys to select a plate size, then press

ENTER to accept the selection.

3. Select

WELL SET.

Select

WELL SET, then press ENTER to accept the selection.

4. Select a well set in the plate.

Use the left and right arrow keys to select the well set, or to move through parameter.
Press

ENTER to confirm the parameter entry.

96-Well

Whole Plate

Edit well Set? (press ENTER)
Waveform: EXP – SQR

---

----

96-Well

Whole Plate

Edit well Set? (press ENTER)
Waveform: EXP – SQR

500

5.500

#. 3 S: 10.0

Plate:
96

Program:
WHOLE PLATE

WELL SET