Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 9)

QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 1
QX200
™
Droplet Digital
™
PCR System
1
1.1 Introduction
The QX200 Droplet Digital PCR (ddPCR ™ ) system performs accurate and
precise digital PCR. The system consists of two instruments, the QX200 droplet
generator and the QX200 droplet reader, and their associated consumables.
The QX200 droplet generator partitions samples into 20,000 nanoliter-sized
droplets and, after PCR on a thermal cycler, droplets from each sample are
analyzed individually on the QX200 droplet reader. PCR-positive and PCR-
negative droplets are counted to provide absolute quantification of target DNA
in digital form. Alternatively, amplified products can be extracted from droplets
following PCR for downstream applications, such as sequencing or cloning.
The ddPCR system lets you:
■ ■
Detect rare DNA target copies with unmatched sensitivity
■ ■
Determine copy number variation with unrivaled accuracy
■ ■
Measure gene expression levels with precision
Applications and uses include:
For instructions on use of the QX200 droplet generator, please refer to the QX200
Droplet Generator Instruction Manual (bulletin 10031907).
■ ■
Copy number variation
■ ■
Rare sequence detection
■ ■
Gene expression analysis
■ ■
Next-generation sequencing (NGS)
library quantification
■ ■
Viral load determination
■ ■
Single cell gene expression analysis
■ ■
Absolute quantification
■ ■
Rare mutant detection
■ ■
miRNA analysis
■ ■
NGS sample preparation
■ ■
GMO detection