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Bio-Rad ddPCR™ Supermix for Probes User Manual

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Chapter 2 Using the QX200 Droplet Reader

QX200 Droplet Reader and QuantaSoft Software Instruction Manual

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3. Place the 96-well PCR plate into the plate holder:

a. Place the 96-well PCR plate containing the amplified droplets into the base of the plate holder. Well A1

of the PCR plate must be in the top left position.

b. Move the release tabs of the top of the plate holder into the “up” position and place the top on the PCR

plate. Firmly press both release tabs down to secure the PCR plate in the holder.

4. Press the button on the green lid to open the droplet reader. Load the plate holder into the droplet reader,

and press the button on the lid again to close the cover. Confirm the first three indicator lights are green
(Table 2.1).

Placing the 96-well plate into the plate holder.

Down

Down

Placing the plate holder into the droplet reader.

Push to open/close

Base of plate holder

96-well PCR plate

Top of plate holder