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5 viewing ratio data (ratio), 6 viewing events – Bio-Rad ddPCR™ Supermix for Probes User Manual

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Chapter 3 Using QuantaSoft Software

3.3.5 Viewing Ratio Data (Ratio)

Click Ratio to view ratio data for selected wells/samples. Use the radio buttons to select a plot of the Ratio
(unkown:reference) or Fractional Abundance (% of sample); select Inverse to apply the inverse of either.

3.3.6 Viewing Events

Click Events to view the number of droplet events counted for selected wells/samples. Use the radio buttons
to select the channels displayed. View positive, negative, or total droplet counts, or any combination of these.

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QX200 Droplet Reader and QuantaSoft Software Instruction Manual

Viewing ratio data. Data from absolute quantification are shown. Hover over data points to reveal well identity, concentration, and Poisson
confidence limits. Select y log to convert the y-axis to logarithmic scale (shown). Solid data points (not shown) indicate merged data; open
data points (shown) indicate data from single wells.

Toggle well, name, or label display

Select ratio or abundance plot, inverse
ratios

Viewing event data. Data from absolute quantification are shown.

Toggle well, name, or label display

Select events to display

Select channel(s) to display