Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 25)

| 17 QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Chapter 3 Using QuantaSoft Software Chapter 3 Using QuantaSoft Software
Channel 1
fluorescence
amplitude vs.
event number
Channel 2
fluorescence
amplitude vs.
event number
Channel 1 (FAM)
histogram of events
vs. amplitude
Channel 2 (VIC or HEX)
histogram of events vs.
amplitude
Viewing channel data for a single well. Processed data from both channels of a single well are shown. In channel 1, the single-well
threshold tool is enabled (the threshold is shown by the pink line and the value in the Set Threshold field).
Channel selector
Viewing channel data for multiple wells. Processed data from both channels of multiple wells are shown. In channel 1, the single-well
threshold tool is enabled (the threshold is indicated by the pink line and the value in the Set Threshold field; the status of that well in the
results also shows Manual). In Channel 2, the multiple threshold tool is enabled.
Threshold adjustment tool
(single well)
Threshold adjustment tools
Reset automatic
threshold settings
Y-axis log scale toggle
Threshold settings
Channel 1
fluorescence
amplitude vs.
event number
Channel 2
fluorescence
amplitude vs.
event number
Channel 1 (FAM)
histogram of events
vs. amplitude
Channel 2
(VIC or HEX)
histogram of events
vs. amplitude
Channel selector
Reset automatic
threshold
settings
Y-axis log scale toggle Threshold settings