Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 10)

QX200 Droplet Reader and QuantaSoft Software Instruction Manual 2 |
Chapter 1 QX200 Droplet Digital PCR System
1.2 System Components
The system consists of two instruments and associated software, consumables, and reagents (Table 1.1):
■ ■
QX200 droplet generator — utilizes proprietary reagents and microfluidics to partition samples into 20,000
nanoliter-sized droplets
■ ■
QX200 droplet reader — following PCR amplification of the nucleic acid target in the droplets, this
instrument analyzes each droplet individually using a two-color detection system (set to detect FAM and
either VIC, HEX, or EvaGreen); PCR-positive and PCR-negative droplets are counted to provide absolute
quantification of target DNA in digital form using QuantaSoft
™ software
Additional materials required for performing ddPCR are listed in Table 1.2.
Table 1.1. QX200 Droplet Digital PCR system components. Items shipped with the QX200 ddPCR system
(catalog #186-4001). Catalog # refers to replacement items (quantities may be different).
Component Description Catalog #
QX200 droplet generator
QX200 droplet generator
Instrument used for droplet generation 186-4002
DG8 ™ droplet generator Microfluidic cartridge used to mix sample and oil to 186-4008
cartridges and gaskets (24) generate droplets; gaskets seal the cartridge to
prevent evaporation and apply pressure required
for droplet formation
Droplet generator cartridge Positions and holds the droplet generator cartridge in 186-3051
holder the instrument for droplet generation
Power cord Connects QX200 droplet generator to power source Call technical support
QX200 droplet reader
QX200 droplet reader
Instrument used for droplet reading, data collection 186-4003
Laptop PC and QuantaSoft software Connects to QX200 droplet reader for data collection 186-3007
and analysis
Droplet reader plate holders (2) Position 96-well PCR plate in the droplet reader 510-10608
USB 2.0 cable Connects QX200 droplet reader to PC Call technical support
Power cord Connects QX200 droplet reader to power source Call technical support
QX200 Droplet Digital PCR system. Left, QX200 droplet reader. Right, QX200 droplet generator.