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2 using the experiment editor – Bio-Rad ddPCR™ Supermix for Probes User Manual

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Chapter 3 Using QuantaSoft Software

Chapter 3 Using QuantaSoft Software

QX200 Droplet Reader and QuantaSoft Software Instruction Manual

Experiment editor. Options for a CNV experiment are shown.

Copy number options appear for CNV
experiments only

Customize the color codes for experiments (for
example, by experiment type, user, etc.)

Settings appear in the Applied Well Settings box as you enter them. When you are done, click Apply or OK to
save the information.

The settings appear in the well in the plate map.

Tips:
You can change the selected well using the plate map without exiting the well editor.
To append sample or assay names with numbers incrementally through selected wells, select
the Auto Inc check box next to Name.
Use standard Windows keyboard shortcuts for copying, pasting, and deleting selections (for
example, use Ctrl+c to copy a selection, Ctrl+z to undo an operation, etc.).

3.1.2 Using the Experiment Editor

Use the experiment editor to define the experiment type. To open the experiment editor, select Experiment
> add experiment... in the well editor or select New or Edit (double-click on an experiment name) in the
Experiments window under Setup. Three types of experiments are possible: Absolute Quantification (ABS),
Rare Event Detection (RED), and Copy Number Variation (CNV). A default list of experiments is supplied at
installation, but you can create and save custom experiments; upgrade installations will preserve current
experiment lists.

Settings are summarized in the Applied Well Settings box as you enter them. When you are done, click Apply
or OK to save the experiment information.

The settings appear in the well in the plate map.

If the Sample Name, Experiment, Assay Name, and Assay Type all match across multiple wells, the software
can identify these as “merged” and provides an option to view merged data during analysis (see Section 3.3).

Click Apply or OK to save the information.

The settings appear in the well in the plate map.