Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 13)

QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 5
Chapter 1 QX200 Droplet Digital PCR System
QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Using the QX200
™
Droplet Reader
2
1. Power on the QX200 droplet reader using the switch at the back. Allow
it to warm up for 30 min, then switch on the PC and launch QuantaSoft ™
software.
2. Check the indicator lights on the front of the droplet reader (Table 2.1). The
first two lights at left should be solid green, indicating power is on, there is
sufficient oil in the designated oil reservoir, and there is <700 ml in the waste
bottle. If the lights are flashing amber, the run cannot be started; clean out
the waste bottle or replace the oil (see Section 4.2.2).
Table 2.1. Status indicator lights on the QX200 droplet reader.
Solid green Power on Bottle levels OK* Plate in place Run complete
Flashing green — Oil <30% or waste >70%** — Run in progress
Flashing amber — Oil <10% or waste >90%*** — Error during run
Off Power off — No plate Idle
* There is sufficient oil and room in the waste bottle to run 96 wells.
** The run can be started if <96 wells are run (for example, only 19% oil is required for 24 wells); if there is not enough oil
for the run, the software will not allow you to start the run.
*** The software will not allow you to start the run.