Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 26)

Chapter 3 Using QuantaSoft Software
3.3.2 Viewing Clustering Plots (2D Amplitude)
Click 2D Amplitude to view the channel 1 vs. channel 2 clustering plot and enable options for manually or
automatically adjusting the thresholds used in assigning positives and negatives for each detection channel.
■ ■
To reset automatic thresholds for positives and negatives, click Auto Analyze
■ ■
To manually assign thresholds:
– Use the thresholding crosshair to assign classification regions for the whole plot (this is the only option in
Heat Map mode)
– Use the ellipse, rectangle, or lasso threshold adjustment tool to classify a region of the plot. Click the tool,
then click the region type in the working cluster selector. Use the tool to select the region within the plot
Tip:
Mouse over any well in the well selector to preview data from that well in the clustering plot.
18 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Viewing clustering plots. Threshold adjustment options available in the clustering plot are shown. Threshold values are indicated by
the pink lines in the plot.
Threshold
adjustment tools
Ch2 threshold
Ch 1
threshold
Working cluster
selector
Ch1+/Ch2– Ch1+/Ch2+
Ch1–/Ch2– Ch1–/Ch2+
Ch1+/Ch2– Ch1+/Ch2+
Ch1–/Ch2+ Ch1–/Ch2–
Reset automatic
threshold settings
Threshold settings
Display
multicolor
heat map