Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 27)

| 19 QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Chapter 3 Using QuantaSoft Software
3.3.3 Viewing Concentration Data (Concentration)
Concentration data for each target appear in the wells in the plate map and are tabulated in the results table.
Click Concentration to visualize data in concentration plots. Use the radio buttons to select the channels
displayed. Error bars reflect total error or Poisson 95% confidence limits. These data can be exported for
analysis in other spreadsheet applications (for example, Microsoft Excel).
3.3.4 Viewing Copy Number Data (Copy Number)
Click Copy Number to view copy number for selected wells/samples.
Toggle y-axis log scale
QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Display data from single wells, merged data, or both Toggle x-axis values and well, name, or label display
Viewing concentration data. Data from absolute quantification are shown. Hover over data points to reveal well identity, concentration,
and Poisson confidence limits. Solid data points (shown) indicate merged data; open data points (not shown) indicate data from single
wells.
Select channel(s) to display
Toggle well, name, or label display
Viewing copy number data. Hover over data points to reveal well identity, concentration, and Poisson confidence limits. Solid data
points (not shown) indicate merged data; open data points (shown) indicate data from single wells.
Y2 Axis options:
None — no Y2 axis
Ch2 — display Ch2 concentration
CNV — display copy number
(CNV analysis only)
Ratio(a/b) — ratio (a/b) of
unknown:reference
Fractional Abundance
(a/a+b) — ratio (a/[a+b]) of
unknown:reference; abundance
Inverse — inverse of ratio or
fractional abundance