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Using quantasoft, Software – Bio-Rad ddPCR™ Supermix for Probes User Manual

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QX200 Droplet Reader and QuantaSoft Software Instruction Manual

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Chapter 2 Using the QX200 Droplet Reader

QX200 Droplet Reader and QuantaSoft Software Instruction Manual

Using QuantaSoft

Software

3

QuantaSoft software organizes and provides one-click access to the three main
steps of droplet analysis in the left navigation bar, moving you through the entire
workflow:

Setup — enter information about the samples, assays, and experiments
(see Section 3.1)

Run — start the run and control the instrument, if needed (see Section 3.2)

Analyze — compute nucleic acid concentration (see Sections 3.3 and 3.4)

QuantaSoft software uses the following file types:

Template (*.qlt) — user-defined plate layout settings (no data) for reading a
ddPCR

plate

Raw data (*.qlb) — unprocessed data from each well in a ddPCR plate

Results (*.qlp) — user-defined plate layout settings and processed data for a
ddPCR plate

Comma-separated values (*.csv) — analyzed data in a format that can be
assessed using other programs, such as Microsoft Excel