Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 30)

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QX100 Droplet Generator Instruction Manual
Chapter 4 Specifications and Maintenance
QX200 Droplet Reader and QuantaSoft Software Instruction Manual 22 |
4.2 Maintenance
4.2.1 General Maintenance Procedures
Surfaces of the instrument may require general cleaning. Use deionized/distilled water for general wipe down
with a slightly dampened cloth. For decontamination, 10% bleach followed by 70% ethanol and/or deionized/
distilled water may be used. Do not use acetone or tap water.
Inspect equipment regularly for damaged external components or wiring. Do not use if damaged.
Apply standard MSDS (Material Safety Data Sheet) and OSHA practices when handling and disposing of
generated waste.
Bio-Rad droplet generation and reader fluids are based on fluorinated hydrocarbon chemistry and should be
disposed of in accordance with institutional, state, and local regulations. These nonflammable fluids are inert
and have low environmental impact and low toxicity. Collect waste in a polyethylene container and discard
within one month.
Droplets made with Bio-Rad master mix have antimicrobial properties, but microbial growth is possible.
The waste profile should contain the following: fluorinated hydrocarbons, water, fluorescent dye (from probes),
protein, and nucleic acids.
4.2.2 Replacing Droplet Reader Oil and Removing Waste
Replace the droplet reader oil and empty the waste receptacle as needed. Use the handle built into the side
compartment to slide the carriage out:
■ ■
Use empty oil supply bottles as new waste bottles. Add 50 ml 10% bleach to the waste bottle to prevent
microbial growth, and place a label on the waste bottle at this time
■ ■
Place the new bottle of oil in the oil position and screw the cap into place. In QuantaSoft ™ software, under
Instrument Routines in the Setup window, click Prime to fill the lines with oil before the system is run
Replacing droplet reader oil and removing waste. Slide out the carriage containing the droplet reader oil and waste receptacle to
remove and replace the bottles (left). After replacing oil, click Prime (right) to fill the lines; other instrument maintenance routines are for
use by field specialists only.