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Bio-Rad ddPCR™ Supermix for Probes User Manual

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QX200 Droplet Reader and QuantaSoft Software Instruction Manual

QX200 Droplet Reader and QuantaSoft Software Instruction Manual

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Chapter 1 QX200 Droplet Digital PCR System

Table 1.2. Additional materials required.

Component

Recommended

Catalog # and Supplier

QX200 droplet generator
Reagents for probe detection
PCR supermix

ddPCR supermix for probes

186-3010, 186-3026, 186-3027,

186-3028

Droplet PCR supermix

186-3023, 186-3024, 186-3025

One-Step RT-ddPCR supermix for probes

186-3021, 186-3022

Droplet generator oil

Droplet generator oil for probes

186-3030, 186-3005

Control

ddPCR buffer control kit for probes

186-3052

Reagents for EvaGreen detection
PCR supermix

QX200

ddPCR

EvaGreen

®

supermix

186-4033, 186-4034, 186-4035,

186-4036

Droplet generator oil

QX200 droplet generator oil for EvaGreen dye

186-4005, 186-4006

Control

QX200 buffer control kit for EvaGreen dye

186-4052

Consumables and other materials
Pipets

20 μl pipet for sample loading

Rainin L-20

50 μl pipet for droplet transfer

Rainin L-50, L8-50

8-channel, 200 μl pipet for oil

Rainin L8-200

Pipet tips

Filtered

Rainin GP-L10F, GP-L200F

96-well PCR plates

twin.tec semi-skirted 96-well plate

Eppendorf 951020362

Reagent trough

Any

Foil plate seals

Pierceable foil plate seals

181-4040

Plate sealer

PX1

PCR plate sealer

181-4000

8-cap strips

Any

QX200 droplet reader
Droplet reader oil

Droplet reader oil

186-3031, 186-3004

Droplet reader waste bottle

Empty waste bottle

N/A