Bio-Rad ddPCR™ Supermix for Probes User Manual
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QX200 Droplet Reader and QuantaSoft Software Instruction Manual
QX200 Droplet Reader and QuantaSoft Software Instruction Manual
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Chapter 1 QX200 Droplet Digital PCR System
Table 1.2. Additional materials required.
Component
Recommended
Catalog # and Supplier
QX200 droplet generator
Reagents for probe detection
PCR supermix
ddPCR supermix for probes
186-3010, 186-3026, 186-3027,
186-3028
Droplet PCR supermix
186-3023, 186-3024, 186-3025
One-Step RT-ddPCR supermix for probes
186-3021, 186-3022
Droplet generator oil
Droplet generator oil for probes
186-3030, 186-3005
Control
ddPCR buffer control kit for probes
186-3052
Reagents for EvaGreen detection
PCR supermix
QX200
™
ddPCR
™
EvaGreen
®
supermix
186-4033, 186-4034, 186-4035,
186-4036
Droplet generator oil
QX200 droplet generator oil for EvaGreen dye
186-4005, 186-4006
Control
QX200 buffer control kit for EvaGreen dye
186-4052
Consumables and other materials
Pipets
20 μl pipet for sample loading
Rainin L-20
50 μl pipet for droplet transfer
Rainin L-50, L8-50
8-channel, 200 μl pipet for oil
Rainin L8-200
Pipet tips
Filtered
Rainin GP-L10F, GP-L200F
96-well PCR plates
twin.tec semi-skirted 96-well plate
Eppendorf 951020362
Reagent trough
Any
Foil plate seals
Pierceable foil plate seals
181-4040
Plate sealer
PX1
™
PCR plate sealer
181-4000
8-cap strips
Any
QX200 droplet reader
Droplet reader oil
Droplet reader oil
186-3031, 186-3004
Droplet reader waste bottle
Empty waste bottle
N/A