beautypg.com

3 analyze – Bio-Rad ddPCR™ Supermix for Probes User Manual

Page 22

background image

Fill in Chapter Name Here on Master Page (33_man_left header/footer)

QX100 Droplet Generator Instruction Manual

Chapter 3 Using QuantaSoft Software

3.3 Analyze

In the Setup window, load a plate (filename.qlp), then click Analyze to open and analyze the data. The data
analysis interface is separated into three windows:

Results table — summarizes results for wells selected in the well selector

Well selector — enables selection of wells for targeted analysis

Processed data/graphical display — allows visualization of graphical data from selected wells

Well data stream

Channel 1 histogram
(events vs. amplitude)

Run indicator

Analyzed wells

Switch data views
(charts to larger
window)

Interface during an active run. Data for both detector channels are shown for the well being read.

Experiment

Conc. channel 1 unknown

Conc. channel 2 unknown

Sample name

Channel 1 (FAM) fluorescence vs. event number

Channel 2 (VIC or HEX) fluorescence vs. event number

Channel 2 histogram (events vs.
amplitude)

14 |

QX200 Droplet Reader and QuantaSoft Software Instruction Manual