Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 3 - Manual (Page 72)

3.24 Conditioning
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940 Professional IC Vario ONE/SeS/PP/Prep 3 (2.940.1530)
5 Installing the output of the separation column
￭ Fasten the column output capillary to the upper end of the sepa-
ration column using a PEEK pressure screw (6.2744.070).
6 Inserting the separation column
￭ Insert the separation column with chip into the column holder
until you hear it snap in place.
The separation column is now detected by MagIC Net.
3.24 Conditioning
After installation, after each time the instrument is switched on and after
each time the eluent is changed, the system has to be conditioned with
eluent long enough that a stable baseline is attained.
NOTE
The conditioning time can lengthen considerably after changing the
eluent.
Conditioning the system
1 Preparing the software
CAUTION
Ensure that the configured flow is not higher than the flow permit-
ted for the corresponding column (refer to the column leaflet and
the chip data record).
￭ Start the MagIC Net computer program.
￭ Open the Equilibration tab in MagIC Net: Work-
place
▶ Run ▶ Equilibration.
￭ Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.
2 Preparing the instrument
￭ Ensure that the column is inserted correctly in relation to the flow
direction marked on the sticker (arrow has to point in the direc-
tion of flow).