Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 3 - Manual (Page 71)

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3 Installation
940 Professional IC Vario ONE/SeS/PP/Prep 3 (2.940.1530)
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Connecting the separation column
1 Removing the stoppers
CAUTION
When inserting the column, ensure that it is inserted correctly
based on the marked flow direction.
￭ Remove the stoppers from the separation column.
2 Installing the input of the separation column
There are 3 possibilities:
￭ Attaching the bottom end of the separation column directly to
the guard column.
or
￭ If the guard column is connected to the separation column using
a provided connection capillary, connect the bottom end of the
separation column to the guard column's output capillary using
the provided PEEK pressure screw (6.2744.070).
or
￭ If no guard column is used (not recommended), connect the col-
umn input capillary to the input of the separation column using a
short pressure screw (6.2744.070).
3 Rinsing the separation column
￭ Place a beaker under the separation column's outlet.
￭ Start manual control in MagIC Net and select the high-pressure
pump: Manual
▶ Manual control ▶ Pump
– Flow: in accordance with column leaflet
– On
￭ Rinse the separation column with eluent for approximately 10
minutes.
￭ Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
4 Removing the coupling
￭ Remove the coupling (6.2744.040) from the column output capil-
lary.