Metrohm 750 Autosampler - Manual (Page 17)

2 Installation
750 Autosampler
14
2.2.4 Tubing connection
For transferring the sample from the 750 Autosampler to the injection
valve of the 733 IC Separation Center the following tubing connections
must be made:
1 Tubing connection 750 – injection valve
• At the 733 IC Separation Center, loosen the rotary nipple
screwed onto the interior side of connection 22 22 or 28 28.
• Take PTFE suction tubing 88 88 (see Fig. 16 and Fig. 17 of the
732/733 Instructions for Use) completely out of connection 22 22
or 28 28 and unscrew from connection "1" of injection valve 68 68.
• Cut transfer tubing 88 (6.1803.000 PTFE capillary tubing of 750
accessories) to the shortest possible length between the
needle of the 750 Autosampler and injection valve 68 68 of the
733 IC Separation Center.
• Remove the cover from transfer needle 55 of the 750 Auto-
sampler and screw one end of transfer tubing 88 to this con-
nection with the aid of a 6.2744.010 PEEK compression fit-
ting.
• Remove holder stopper 77 from holder 66 and pull 6.1803.000
PTFE capillary tubing so far through the opening of holder
stopper 77 that the tubing length between needle connection
and the holder stopper is about 30 cm. Then press holder
stopper 77 fully back into holder 66.
• Pull the other end of the transfer tubing 88 through the opening
22 22 or 28 28 of the 733 IC Separation Center and screw onto
connection "1" of injection valve 68 68 using a 6.2744.010 PEEK
compression fitting.
• Retighten rotary nipple on the interior side of connection 22 22
or 28 28 to fix the capillary tubing.
2 Tubing connection injection valve – waste
• Insert 6.2744.020 coupling (from 733 accessories) into con-
nection 21 21 or 27 27 of the 733 IC Separation Center.
• Screw PTFE suction tubing 88 88 onto the 6.2744.020 coupling
attached to connection 21 21 or 27 27 and lead it into the waste
container.
In the case of the 2.733.0X20 IC Separation Center with two injection
valves, it is possible to fill both sample loops from the same 750
Autosampler. For this, connection "1" of valve A (outlet of the sample
loop) must be connected to connection "2" of valve B (inlet of the
sample loop) using a 6.1803.000 PTFE Capillary tubing cut to the
appropriate length.