Metrohm 754 Dialysis Unit User Manual

Page 18

2.3 Attaching the accessories

754 Dialysis Unit

Pump tubings are consumable material with a lifetime which depends

on the contact pressure. This is why the tubing cartridges should be

raised completely by loosening snap-action lever 88 on the right-hand

side if the pump is to remain switched off for a considerable length of

time (the set contact pressure remains unchanged).

The 6.1826.0X0 pump tubing is made of PVC and must not be used

for rinsing with solutions which contain acetone. In such cases, rinse

with different pump tubing or a different pump.

2.3.4

Assembling the dialysis cell

A dialysis membrane has to be inserted before the dialysis cell can be

placed inside the 754 Dialysis Unit. Proceed as follows (see Fig. 7):

1 Prepare dialysis cell

•

Extract dialysis cell 34

34 (6.2729.100) from its packaging and

remove the four 6.2744.060 dummy stoppers.

•

Using the 6.2621.070 Allen key, completely loosen the 5

screws 39

39, separate top part 38

38 from bottom part 35

35 and re-

move sealing ring 36

36.

•

Thoroughly rinse the sealing ring 36

36, bottom part 35

35 and top

part 38

38 of the dialysis cell with ultrapure water and dry with N

or a lint-free cloth.

Use only ultrapure water or ethanol to clean the dialysis cell;

organic solvents (e.g. acetone) will cause damage to the plexiglas

cell !

2 Prepare dialysis membrane

•

Use the 6.2831.010 pincette to extract a new dialysis mem-

brane 38

38 (e.g. 6.2714.010) from its packaging and immerse

in a Petri dish containing ultrapure water for approx. 2 min

until the membrane is completely saturated with water.

3 Insert dialysis membrane

•

Lay bottom part 35

35 on a paper wipe with its inside facing

upwards.

•

Insert sealing ring 36

36 in the appropriate recess in bottom part

35.

•

Using the 6.2831.010 pincette, place the wet dialysis mem-

brane 38

38 (e.g. 6.2714.010) inside the sealing ring 36

36 on the

bottom part 35

35.

Once saturated, take care that the dialysis membrane is not allowed to

dry out, otherwise it cannot be used any more !