Leica Biosystems CM1520 - Manual (Page 89)

89 Leica CM1520 – Cryostat
10.2 Mobile heat extractor – application
Specimen freezing with the freezing shelf can be accelerated by the ad-
ditional use of a heat extractor.
• Store the heat extractor in the cryochamber.
• Place it on the specimen surface to accelerate freezing.
• Remove it once the specimen is entirely frozen.
10. Ordering Information, Components and Consumables
Fig. 118
7
6
Fig. 119
• Place the cap (9) on the required side, so that
the appropriate location hole for the specimen
disc is visible.
• Fit the pin (6) of the specimen disc (5) into the
corresponding bore (7) on the cold extractor.
• After about 20 seconds, the frozen specimen
can be removed from the specimen disc with
forceps.
• If the cap is too loose, readjust it with the small
screw (10).
Do not overtighten the screw.
• After removing the specimen, remove the cold
extractor from the cold cryochamber once
again.
9
8

5

10

Position the speci-
men here using
forceps
Keep the thermal block outside the cryochamber at room tempera-
ture. Place it in the cryochamber only for specimen removal.
10.3 Cold extractor – application
The thermal block (8) facilitates the removal of the frozen specimen from
the specimen disc.
Recommendation:
It is recommended to precool the heat extractor in liquid nitrogen or
other refrigerant.