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Leica CM1520 – Cryostat
7.
Daily Use of the Instrument .................................................................................................................. 31
7.1
Specimen freezing ..................................................................................................................................... 31
7.1.1 Quick freeze shelf ....................................................................................................................................... 31
7.1.2 Stationary heat extractor
(optional) ............................................................................................ 32
7.2
Specimen discs .......................................................................................................................................... 32
7.2.1 Inserting the specimen discs into the specimen head ........................................................................ 32
7.2.2 Specimen orientation ................................................................................................................................ 32
7.3 Sectioning .................................................................................................................................................... 33
7.3.1 Blade holder CE .......................................................................................................................................... 33
7.3.2 Blade holder CE-TC .................................................................................................................................... 39
7.3.3 Knife holder CN ........................................................................................................................................... 40
7.3.4 Knife holder CN-Z with anti-roll guide .................................................................................................... 44
7.3.5 Cleaning the blade and knife holders .................................................................................................... 44
7.3.6 Installing the shelf (movable) (optional) ................................................................................................. 46
7.4
Temperature selection chart (in minus °C) ............................................................................................ 47
7.5 Defrosting .................................................................................................................................................... 48
7.5.1 Automatic defrosting of the cryochamber ............................................................................................. 48
7.5.2 Manual defrosting of the cryochamber .................................................................................................. 48
7.5.3 Manual defrosting of the quick freeze shelf .......................................................................................... 49
7.6
Terminating work ....................................................................................................................................... 49
7.6.1 Terminating daily work .............................................................................................................................. 49
7.6.2 Shutdown for a longer period .................................................................................................................. 50
8. Troubleshooting ..................................................................................................................................... 51
8.1
Error messages in the display .................................................................................................................. 51
8.2
Temperature control button ..................................................................................................................... 52
8.3
Possible sources of error, causes and remedies ................................................................................. 53
8.3.1 Changing the battery ................................................................................................................................. 57
9.
Cleaning, Disinfection, Maintenance ................................................................................................ 58
9.1 Cleaning ....................................................................................................................................................... 58
9.2
Spray disinfection with Leica Cryofect ................................................................................................... 59
9.3 Maintenance ............................................................................................................................................... 60
9.3.1 General maintenance instructions .......................................................................................................... 60
9.3.2 Replacing the fuses ................................................................................................................................... 62
9.3.3 Replacing the fluorescent lamp ............................................................................................................... 63
10.
Ordering Information, Components and Consumables ................................................................... 64
10.1 Ordering information .................................................................................................................................. 64
10.2 Mobile heat extractor –
application ............................................................................................ 89
10.3 Cold extractor –
application ....................................................................................................... 89
11.
Warranty and Service ........................................................................................................................... 90
12.
Decontamination Certificate (master) ............................................................................................... 91
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