Leica Biosystems CM1520 - Manual (Page 54)

54 Instructions for Use V1.3 – 10/2012
8. Troubleshooting
Problem Cause Remedy
Sections not properly flattened
despite correct temperature and
correctly aligned anti-roll plate.
- Knife/blade and/or anti-roll plate
dirty.
- Edge of anti-roll plate damaged.
- Blunt knife/blade.
- Clean with dry cloth or brush.
- Replace plate.
- Use different part of the cut-
ting edge or replace.
Sections curl on the anti-roll plate. - Anti-roll plate does not protrude
far enough beyond the cutting
edge.
- Readjust correctly.
Scraping noise during sectioning
and specimen return movement.
- Anti-roll plate protrudes too far
beyond the cutting edge and is
scraping against the specimen.
- Readjust correctly.
Uneven sections. - Knife/blade damaged.
- Edge of anti-roll plate damaged.
- Use different part of the cut-
ting edge or replace.
- Replace plate.
Chatter during sectioning. - Specimen insufficiently frozen
onto the specimen disc.
- Specimen disc not clamped
tightly.
- Specimen holder ball joint not
clamped.
- Knife/blade not clamped tightly
enough.
- Specimen has been sectioned too
thickly and has detached from the
disc.
- Very hard, inhomogeneous speci-
men.
- Blunt knife/blade.
- Knife profile inappropriate for this
specimen used.
- Incorrect clearance angle.
- Refreeze specimen onto the
disc.
- Check specimen clamping.
- Check ball joint clamping.
- Check knife/blade clamping.
- Refreeze specimen onto the
disc.
- Increase section thickness;
reduce specimen surface
area if necessary.
- Use different part of the cut-
ting edge or replace.
- Use knife with different pro-
file.
- Set correct clearance angle.