Leica Biosystems CM1520 - Manual (Page 49)

49 Leica CM1520 – Cryostat
7.6 Terminating work
7.6.1 Terminating daily work
7. Daily Use of the Instrument
To avoid frost formation always put the cover on the quick freeze shelf. Always cover the
quick freeze shelf during breaks and overnight.
7.5.3 Manual defrosting of the quick freeze shelf
The quick freeze shelf can become hot during the defrosting process. Therefore, do not
touch it!
In case of heavy frost formation on the quick freeze shelf, which can occur particularly
after the spray disinfection, a defrost can be activated manually ( see point 6.2.4 ). which
can be terminated when required.
• Remove all the specimens from the cryostat.
• Attach the cover for the freeze shelf.
• Remove the plug and close the sliding window.
• Turn out the cryochamber illumination.
• Lock control panel field 1 (
Fig. 14 ) using the key button.
• Do NOT switch off the instrument using the circuit breaker, as otherwise no cooling takes
place.
Only common detergents and alcohol-based disinfectants should be used for cleaning.
All components removed from the cold environment will collect condensation. Therefore,
dry them thoroughly before placing them back into the cryochamber.
• Lock the handwheel.
• Take the knife/blade out of the knife/blade holder and put it back into the knife box/blade
dispenser in the cryochamber.
• Remove frozen section waste with a cold brush.
• Empty the section waste tray.
• Clean the storage shelves and brush shelf.