Troubleshooting, Problem cause remedy – Leica Biosystems CM1520 User Manual
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Instructions for Use V1.3 – 10/2012
8. Troubleshooting
Problem
Cause
Remedy
Tissue adheres to the anti-roll
guide.
- Dried specimen.
- Anti-roll plate is too warm or
incorrectly positioned.
- Static charge.
- Fat on the corner or edge of anti-
roll plate.
- Rust on the knife.
- Cool down anti-roll plate or
reposition correctly.
- Remove static electricity.
- Remove fat with alcohol.
- Remove rust.
Flattened sections curl up when
anti-roll plate is folded up.
- Static charge or air current.
- Anti-roll plate too warm.
- Remove static electricity.
- Cool down the anti-roll plate.
Sections tear or separate.
- Temperature too low for the tis-
sue being sectioned.
- Blunt part, dirt, dust, frost or rust
on the knife.
- Front edge of the anti-roll plate
damaged.
- Hard particles in the tissue.
- Dirt on back of knife.
- Increase temperature and
wait.
- Remedy cause (--> see indi-
vidual specific solution).
- Replace plate.
- If the application permits, cut
deeper during sectioning.
- Clean.
Inconsistent or insufficient speci-
men feed.
- Defective microtome.
- Call technical service.
Specimen disc cannot be removed. - Moisture on the underside
causes the specimen to freeze
to the freezing shelf or specimen
head.
- Apply concentrated alcohol
to the contact point or heat
the specimen head.
Cryostat non-operational.
- Mains plug not properly con-
nected.
- Circuit breaker is switched off.
- Check if power plug is prop-
erly connected.
- Move the switch back to the
upper click stop.