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Troubleshooting, Problem cause remedy – Leica Biosystems CM1520 User Manual

Page 56

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56

Instructions for Use V1.3 – 10/2012

8. Troubleshooting

Problem

Cause

Remedy

Tissue adheres to the anti-roll

guide.

- Dried specimen.

- Anti-roll plate is too warm or

incorrectly positioned.

- Static charge.

- Fat on the corner or edge of anti-

roll plate.

- Rust on the knife.

- Cool down anti-roll plate or

reposition correctly.

- Remove static electricity.

- Remove fat with alcohol.

- Remove rust.

Flattened sections curl up when

anti-roll plate is folded up.

- Static charge or air current.

- Anti-roll plate too warm.

- Remove static electricity.

- Cool down the anti-roll plate.

Sections tear or separate.

- Temperature too low for the tis-

sue being sectioned.

- Blunt part, dirt, dust, frost or rust

on the knife.

- Front edge of the anti-roll plate

damaged.

- Hard particles in the tissue.

- Dirt on back of knife.

- Increase temperature and

wait.

- Remedy cause (--> see indi-

vidual specific solution).

- Replace plate.

- If the application permits, cut

deeper during sectioning.

- Clean.

Inconsistent or insufficient speci-

men feed.

- Defective microtome.

- Call technical service.

Specimen disc cannot be removed. - Moisture on the underside

causes the specimen to freeze

to the freezing shelf or specimen

head.

- Apply concentrated alcohol

to the contact point or heat

the specimen head.

Cryostat non-operational.

- Mains plug not properly con-

nected.

- Circuit breaker is switched off.

- Check if power plug is prop-

erly connected.

- Move the switch back to the

upper click stop.