Leica Biosystems CM1520 - Manual (Page 31)

31 Leica CM1520 – Cryostat
7. Daily Use of the Instrument
7.1 Specimen freezing
• Select the sectioning temperature (chamber temperature) according to
the tissue type to be sectioned ( see "7.4 Temperature selection chart" ).
7.1.1 Quick freeze shelf
The cryochamber is equipped with a quick freeze shelf (5, Fig. 23 ) on which
up to 10 specimen stages with specimens can be attached.
The temperature of the quick freeze shelf is always lower than the cryo-
chamber temperature.
• Cut the specimen to size.
• Apply a sufficient amount of cryocompound
to a specimen disc to orient and mount the
specimen.
• Place the specimen on the disc and orient.
• Place the specimen disc in one of the holes of
the quick freeze shelf and freeze the specimen
at a low temperature.
• Once the specimen is frozen, insert the speci-
men disc ( Fig. 24 ) with the specimen into the
specimen head ( Fig. 24 ) and start sectioning.
Fig. 23
1
2
5
3