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6 care, sterilization and maintenance, 1 care, 2 sterilization – Eppendorf Research pro User Manual

Page 27: 6 care, sterilization and maintenance 6, Care, sterilization and maintenance

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The outside of the pipette can be wiped with a moist cloth. The use of water with cleaning
wetting agent is also permitted.

Do not allow any liquid to enter the pipette!

After that, the lower part only of the pipette should be rinsed with distilled water and dried. The
pipette may be carefully wiped clean with Isopropanol. The procedure for replacing defective
O-rings (when the pipette tips fit incorrectly) is described in Sec. 6.3.

If the pipette is severely contaminated or if very aggressive chemicals are dispensed, the lower
half of the Research pro should be disassembled (see Sec. 6.2 for the single-channel model and
Part B: "Ordering information" for the multi-channel model). The individual parts are rinsed in
distilled water and then dried. The piston is then lightly lubricated using silicone grease.

Prior to assembling the pipette, check that the piston is positioned correctly.
Following reassembly, a motor reset must take place. This is effected by holding
down the Reset key until a

appears in the display and the motor starts.

Only the lower half of the pipette can be steam-autoclaved (121 °C, 1 bar

*

overpressure,

20 minutes). The autoclaved parts must be allowed to dry at room temperature. The entire lower
part of the multi-channel model can be autoclaved as one unit. With the single-channel model,
the individual parts of the lower part must be autoclaved separately.

Lower part of multi-channel model
Disassembly:

Disassembly of old type of "construction":

Pull the lower part downwards slowly. The multi-channel piston, which is held in place
magnetically, jerks sharply and then comes off.

*

(1 bar 100 kPa 14.5 psi)

6.1 Care

R

6.2 Sterilization

R

Hold down ejector.

Then rotate lower part
clockwise until the
lower part separates
from the pipette.

Piston

Opening
for metal pin

To loosen the lower part of the multi-
channel pipette, rotate it in the arrow
direction which is normally not
permitted.

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Care, sterilization and maintenance

6 Care, sterilization and maintenance

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E06 Pflege etc. Seite 27 Dienstag, 19. Dezember 2006 2:16 14