Eppendorf Research pro - Manual (Page 15)

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Note: The MAN (manual) option is the equivalent of operation with a mechanical piston-stroke
pipette. Liquid is aspirated and dispensed only when the Actuate key is held down. The volume
display increases the number of applications available in comparison to a mechanical pipette:
1. Aspirating: Measuring a small, unknown amount of liquid via aspiration into the pipette tip.
The volume of aspirated liquid appears in the display. If necessary, dilute the liquid to the
defined end volume by aspirating additional liquids. Liquids can be separated by aspirating
air bubbles. The display shows the total volume.
2. Dispensing: Titration is carried out. The amount of liquid dispensed appears in the display.
There is no blow-out.
Setting lower speeds is strongly recommended.
Programming
Carrying out liquid aspiration
If PROG appears in
the display: Press
the
key
briefly until PROG
disappears.
Select "PIP" for pipetting.
Volume selection
(here: 1,000 µL).
The volume rocker has two
pressure points for fast or slow
volume adjustment for up and
down.
If necessary, press the Option
key several times, until MAN
appears in the display. MAN is
selected.
If appears in the display: Press the key briefly.
Liquid is aspirated. The
aspirated liquid appears in the
display.
The direction is changed using
the volume rocker.
Volume correction,
e.g. for removing an air bubble.
5.7 Pipetting with the MAN option
Select
Program
Volume
Option Option Option
R R
Volume
Operation
5 Operation
5
E05_1 Bedienung A5 Seite 15 Dienstag, 19. Dezember 2006 2:05 14