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5 operation – Eppendorf Research pro User Manual

Page 22

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SDI = Serial Diluting

For serial diluting, a defined volume of liquid is aspirated into the pipette tip. This liquid is
dispensed and then mixed with a specific liquid volume by means of a programmable mixing
process (volume, mixing cycles). The mixing procedure (MIX) is started by pressing the Actuate
key separately.

ASP = Aspirate

(multiple aspiration of one volume)

In this program, a defined volume can be aspirated several times. If multiple aspiration of a
volume is complete, the filled pipette tip is emptied. This procedure is the equivalent to a
"reverse dispensing". The program may be used for exchanging nutrient solutions.

EDIT

If

PROG

is in the display, the programming level can be opened by

holding down

the Program

key. The Program key must be held down until the word

EDIT

appears in the display in front of

PROG 1 – 5

. Step-by-step programming is explained in the following sub-section.

If the programming of a program sequence is quit by pressing RESET, the entries for the
program sequence are not stored.
The same program sequence – with, for example, different volumes or options – can be stored
in several memory slots. It would therefore be possible for different pipetting sequences to be
stored permanently in the program level.
Program sequences can be overwritten at any time under

EDIT

(Hold down the Program key).

During the programming process, the entry which is to be defined flashes in the display.
Selections are made using the Select rocker. Volumes are selected using the Volume rocker.

Data is stored by pressing the Actuate key (ENTER function).
Options and speeds can be selected in the programming level by using the Select rocker or by
using the Option or Speed key. Descriptions of how to use the Option and Speed keys can be
found in Secs. 5.2 – 5.9. We recommend that you read these sections before you start
programming. The following section on programming for pipetting contains only a description of
operation using the rockers. Programming and execution of the other programs is contained in
Part B of this manual, which is printed in English and Spanish.

5 Operation

Operation

5

E05_2 Bedienung A5 Seite 22 Dienstag, 19. Dezember 2006 2:07 14