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Aspirating and dispensing speeds, Loading gels, 5 operation – Eppendorf Research pro User Manual

Page 10: Operation

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10

Aspirating and dispensing speeds
The selected speed can be viewed by pressing the Speed key several times. The speed is
changed as follows:

*

This procedure does not necessarily have to be completed using the Speed key. Any key –
with the exception of Reset – may be used. If a key other than the Speed key is used to
complete the procedure, the function of this key is also executed at the same time.

The direction of the arrow for speed flashes in this input field.
The speed can be altered before every dispensing or aspiration.
Different speeds can already be stored for the three basic modes (PIP, DIS, FIX).

Important! High speeds

have a much lower current consumption than low

speeds.

Loading gels
With the 10 µL and 100 µL Research pro, the lowest speeds for liquid dispensing

are ideal

for loading gels. To enable users to work rapidly and with a low electricity consumption, the
highest possible speed for liquid aspirating

should be used. Depending on the task at hand,

gels can be loaded in the Standard mode (Sec. 5.3) or by using the BLO (Sec. 5.4) or MAN
(Sec. 5.7) options. Using the BLO option makes it possible to discharge the blowout outside of
the gel pouch, thus preventing turbulence in the gel pouch.

Motor-
reset

If

is held down until a piston movement begins, a motor reset is effected.

This reset routine lasts roughly 10 seconds.
The accuracy of the piston movement is ascertained with the motor reset.

The Research pro has two operating levels. The basic modes for pipetting (PIP and
FIX) and dispensing (DIS) are accessed in the first level by pressing the Select rocker.
PROG appears in the display by double-clicking the "Program" key. You are now in
the second level (program level). Using the Select rocker, select from the programs
(Sec. 5.10). This level can be quit by pressing the "Program" key briefly once. PROG
disappears from the display.

Please note the following descriptions of the symbols used in the program sequence:

An Actuate key which has
been pressed briefly is light.

An Actuate key which has
been held down is dark.

If the piston movements appear in the display without any volumes, this indicates a
process which is not connected to liquid aspiration or dispensing.

Sequence

for pressing keys

Magnifying glass indicates
important display information

Comment

Display and selection of
aspirating speed.

Display and selection of
dispensing speed.

R

Program

Speed

Select

Speed

Select

Speed

*

5 Operation

Operation

5

E05_1 Bedienung A5 Seite 10 Dienstag, 19. Dezember 2006 2:05 14