6 reverse pipetting (rp), 5 operation, Operation – Eppendorf Research pro User Manual
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RP is recommended for solutions with a high viscosity or with a slightly higher vapor pressure.
"Reverse Pipetting" is liquid aspiration with a blow-out. The liquid is dispensed without a blow-
out. If Filtertips are used, please observe the volume restriction (Sec. 5.11).
Programming
Procedure
If PROG appears in
the display: Press
the
key
briefly
until PROG
disappears.
Select "PIP" for pipetting.
Volume selection
(here: 800 µL). The volume
rocker has two pressure points
for fast or slow volume
adjustment for up and down.
If necessary, press the Option
key several times, until RP
appears in the display. RP is
selected.
If
appears in the display: Press the
key briefly.
Aspiration of liquid with blow-
out.
Liquid is dispensed.
Blow-out is discarded.
If necessary, replace the tip.
Pipette is once again ready to
aspirate liquid with blow-out.
If the Actuate key is held down
when liquid is dispensed, the
next liquid aspiration without
blow-out takes place when the
key is released.
The liquid from the blow-out
may continue to be used.
5.6 Reverse Pipetting (RP)
Select
Program
Volume
Option
Option
Option
R
R
Alternative procedure for dispensing and aspirating
5 Operation
Operation
5
E05_1 Bedienung A5 Seite 14 Dienstag, 19. Dezember 2006 2:05 14