2 information on initialization (ini), 5 operation, Operation – Eppendorf Research pro User Manual

Page 26

Examples and notes for volume correction (balance in mg; density in mg/µL = g/mL).
1.

An actual volume of 98 µL was calculated using formula

(1).

The display volume is 100 µL.
In this case, the following formula should be used:

The value 1.02 is entered under "m". This means that, although 100 µL appears in the
display, the piston makes a stroke movement which is larger by a factor of 1.02, so that
100 µL of this liquid can be aspirated.
If only 100 µL of this liquid is dispensed in every case or if the same factor is produced when
other volumes are tested, a correction of "m" only is sufficient.

If greatly differing factors are produced for different volumes, it is advisable to carry out a
correction of axis section "b" and gradient "b". This requires a calculator with the "linear
regression" statistics function [y=mx+b]. The data for "m" and "b" are calculated by entering
the actual volume (x) and the display volume (y). The actual volume (x) and the display
volume (y) must consist of at least two pairs of different volumes, each of which differ
greatly.. Linear regression can also be carried out using PICASO II.

Following programming, the calculated data must then be checked via weighing
and by using formula (1).

Note: In the case of mechanical pipettes, only axis section "b" can be corrected.

The calibration data can be changed as follows:
–

Call up the device parameters (see 5.11).

–

Proceed using "ENTER" until "CAL 0.0 µL b" is entered.

–

Hold down the "Program" key until the number flashes.

–

Set the desired number using the "Volume" or "Select" key.
The

CAL

symbol appears when b

≠

0.0.

–

Confirm the number selected by pressing "ENTER".

–

The entry for "m" flashes. This can also be changed by pressing "Volume" or "Select".
Entries are stored by pressing "ENTER".

–

The ranges for "m" and "b" differ slightly according to the volume size of the pipette.

If the Research pro is to be used at another workstation and if all volumes and programs
defined by the user should no longer be used, the pipette can be initialized as per delivery
package by means of INI.

Following initialization all user settings and programs are deleted!

Initialization is carried out as follows:

–

Using "ENTER", access "INI" in the device parameters.

–

Hold down the "Program" key.

–

Initialization is completed when the device parameter "INI" disappears from the display.

Display volume 100 µL

Actual volume 98 µL

1.02

5.11.2 Information on initialization (INI)

5 Operation

Operation

E05_2 Bedienung A5 Seite 26 Dienstag, 19. Dezember 2006 2:07 14