Eppendorf Research pro - Manual (Page 21)

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To reach the programming level, the key must be double-clicked.
PROG appears in the display together with a memory slot number from 1 – 5 .
With the aid of the rocker, five programs can be shown in the left-hand side of the display.
To program the five memory slots, it is possible to select from seven different program
sequences:
PIP = Pipetting
As per PIP outside the program level. The complete sequence with volume, option and speeds
is programmed in one memory slot. After programming has been completed, the procedure
can
no longer be changed by repeatedly pressing the keys or the volume rocker. This is also
applicable for the other program sequences.
SP = Sequential Pipetting
Up to ten different pipettings can be linked up to each other. Volume, option and speeds are
defined for each pipetting sequence.
The MAN option is not available here.
DIS = Dispensing
As per DIS outside the program level. The complete procedure is programmed in one memory
slot with dispensing volume, dispensing steps and speeds.
The use of filter tips is not particularly recommended!
The dispensing procedure was changed as of V.1.56.
Prior to this version the liquid is directly dispensed following the automatic return stroke.
SD = Sequential Dispensing
Up to 20 dispensing steps can be defined. Each dispensing step may have a different partial
volume. The total volume may exceed the filling level of a pipette tip.
This means that new liquid for the pipette tip must be aspirated during the dispensing
procedure. Only one aspirating and dispensing speed is defined for all dispensing steps.
The use of filter tips is not particularly recommended!
Changed procedure as of software version V.1.56! See description for DIS.
ADS = Automatic Dispensing
With the Actuate key held down, all dispensings are executed automatically with the same
volume and in a user-defined rhythm (0.1 – 10.0 seconds). Automatic dispensing may be
interrupted by releasing the Actuate key. Apart from this, the procedure is identical to that of
DIS. The use of filter tips is not particularly recommended!
Changed procedure as of software version V.1.56! See description for DIS.
DIL = Diluting
During the diluting process, a diluent, then an air bubble and finally a sample are aspirated into
the pipette tip. The diluent volume and the sample volume are defined by the user. The air
bubble is dependent on the sample volume and is always specified specimen by the program.
The entire contents of the pipette tip can be dispensed by selecting a pipetting option.
Depending on the pipette used, the dilution ratio is between approximately 1+1 and 1+48
(applies as of V.1.56 and data set 2).
5.10 Notes on the programs
Program
Select
Operation
5 Operation
5
E05_2 Bedienung A5 Seite 21 Dienstag, 19. Dezember 2006 2:07 14