Eppendorf BioPhotometer plus - Manual (Page 47)

47
11 Evaluation procedure
BioPhotometer plus — Operating manual
Calculation of the molar concentration
Application: calculation of the molar concentration of the nucleic acid from the mass
concentration and relative molar mass. The molar mass is either entered directly or calculated by
the device from the entered figure of the bases or base pairs per nucleic acid molecule.
C
Mol
= calculated molar concentration of the nucleic acid; the unit for the molar concentration is
programmed in the parameter list of the method; dependent on the programmed unit the formula
above is adjusted automatically.
C = mass concentration of the nucleic acid in ng/μl or μg/ml, from the measured absorbance the
device calculates the concentration result and displays it on the results screen
MM = relative molar mass in kDa
If in the third results screen the number of bases or base pairs per nucleic acid molecule has
been entered instead of the relative molar mass, then the molar mass is calculated from the
number of bases (or pairs):
For dsDNA:
For ssDNA, RNA, Oligo:
MM = calculated relative molar mass in kDa
bp = entered number of base pairs per molecule
b = entered number of bases per molecule
EN
O
perating manual