Eppendorf BioPhotometer plus - Manual (Page 16)

5 Operation
16
BioPhotometer plus — Operating manual
5.2 Methods
The following methods are available and already preprogrammed ex factory. You can modify
most parameters and save them as a modified method (see Parameter on page 26).
Method group Method Explanation Wavelength
DNA dsDNA Calculating the concentration of DNA with
evaluation via factor. The methods differ
mainly in the preprogrammed factor. Measuring wavelength: 260 nm
Secondary wavelengths to check
for purity: 230, 280, 340 nm
ssDNA
OLIGO DNA
RNA RNA Analogous to method group DNA. As method group DNA.
OLIGO RNA
Protein BCA Calculating the concentration of proteins after
adding reagent. The methods are
preprogrammed with the evaluation procedure
calibration curve. Number and target
concentration of the standards can be
modified. 550 nm
BCA MICRO
BRADFORD
595 nm
BRADFORD MICRO
LOWRY
595 nm
LOWRY MICRO
PROTEIN 280 nm
Calculating the concentration of proteins with
evaluation via factor. Measuring wavelength: 280 nm
Secondary wavelengths to check
for purity: 260, 340 nm
OD600 OD600 Turbidity measurement to determine the
bacteria density. 595 nm
dye 550 DYE 550-dsDNA For dye-labeled biomolecules: calculating the
concentration of the molecule (nucleic acid or
protein) and the dye in a single measuring
procedure. The frequency of incorporation of
the dye in the biomolecule is also determined. DNA/RNA/OLIGO: see method
groups DNA and RNA
PROTEIN: see method
PROTEIN 280 nm
Measuring wavelength for the
dye: 550 nm
DYE 550-ssDNA
DYE 550-RNA
DYE 550-OLIGO
DYE 550-PROTEIN
dye 650 DYE 650-dsDNA Analogous to method group "dye 550". DNA/RNA/OLIGO: see method
groups DNA and RNA
PROTEIN: see method
PROTEIN 280 nm
Measuring wavelength for the
dye: 650 nm
DYE 650-ssDNA
DYE 650-RNA
DYE 650-OLIGO
DYE 650-PROTEIN
assay 340 ASSAY 340/1 Calculating the concentration by measuring at
340 nm. The evaluation procedures can be
freely programmed. As a sample the methods
are already preprogrammed with the following
evaluation procedures:
340/1: evaluation via factor
340/2: evaluation via a standard.
340/3: evaluation via calibration curve with 6
standards. 340 nm
ASSAY 340/2
ASSAY 340/3
assay 405 ASSAY 405/1 Analogous to method group "assay 340". 405 nm
ASSAY 405/2
ASSAY 405/3
assay 490 ASSAY 490/1 Analogous to method group "assay 340". 490 nm
ASSAY 490/2
ASSAY 490/3
ABSORBANCE ABSORBANCE Rapid absorbance measurement after
selecting the wavelength. 230, 260, 280, 340, 405, 490,
550, 595, 650 nm
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