Eppendorf BioPhotometer plus - Manual (Page 26)

6 Parameter and functions
BioPhotometer plus — Operating manual
26
6 Parameter and functions
6.1 Parameter
The methods of the BioPhotometer plus as well as the corresponding parameters have already
been preprogrammed ex factory. You can modify these parameters.
6.1.1 View, change and store the parameters of a method
1. Select the desired method group using the corresponding method key.
2. Using the cursor keys select the desired method.
3. Using parameter/dilution open the parameters for this method.
4. Scroll through the parameter list with the cursor keys.
5. Modify the parameters, if necessary, and confirm the changes using Enter.
There are two kinds of parameter entries:
• Selection parameters: selection via cursor keys.
• Numerical values: input via number keys.
6. Exit the parameter list by pressing parameter/dilution again.
You return to the method selection.
6.1.2 Summary and description of parameters
Hint!
Each parameter entered is only saved after confirmation using the Enter key.
Parameter Entry Explanation
Cuvette Selection:
10, 5, 2, 1 or 0.2 mm Optical optical path length of the cuvette. The factor for
converting the absorbance into the concentration is
corrected accordingly internally.
Unit Selection:
Different units dependent on
the method. Result unit for the method.
In some methods this selection is missing because a unit
has been preprogrammed permanently.
MOL. Unit Selection:
Different units dependent on
the method. Molar unit, only for the nucleic acid methods: for the
conversion of concentration units into molar concentrations.
CALCULATION Selection:
• STD
• FACTOR
Evaluation procedure for calculating the sample
concentration:
• with fixed factor
• with standards (calibration)
Factor Numerical input (5 digits) Factor for converting absorbance into concentration. The
number of digits after the decimal point of the factor
determines the number of digits after the decimal point for
the result.
The factor must be entered for a cuvette optical path length
of 10 mm.
STD NUMBER 1-10 Numerical input (1 to 10) Number of standards with different concentrations used for
the calibration.
STD MEASUREMENT Selection:
1x, 2x or 3x Repeated measurements of standards.
EN
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