Eppendorf BioPhotometer plus - Manual (Page 29)

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6 Parameter and functions
BioPhotometer plus — Operating manual
Method group "dye 550"
The unit for the dye is defined permanently with "pmol/μl" and can therefore not be programmed
in the parameters.
For the various dyes the following dye-specific values have been preprogrammed as absorbance
coefficients and correction factors (for the parameter CORRECTION A
550
):
The values for ALEXA546 were not known at the time of writing this manual; please contact the
manufacturer (Invitrogen) with regard to these values.
Method group "dye 650"
The parameters correspond mainly to those of method group "dye 550". As preselection for the
dye Cy 5 has been programmed. For the dye-specific values the following applies:
Parameter DYE 550-dsDNA DYE 550-ssDNA DYE 550-RNA DYE 550-OLIGO DYE
550-PROTEIN
CUVETTE 10 mm 10 mm 10 mm 10 mm 10 mm
Unit
(only for nucleic
acid) μg/mL μg/mL μg/mL μg/mL mg/mL 
(protein: no entry,
programmed
permanently)
MOL. UNIT
(for nucleic acid) pmol/mL pmol/mL pmol/mL pmol/μL
FACTOR
(for nucleic acid or
protein) 50.0 37.0 40.0 30.0 -----
CORRECTION NO
CORRECTION NO
CORRECTION NO
CORRECTION NO
CORRECTION NO
CORRECTION
Dye selection CY3 CY3 CY3 CY3 CY3
ABS.COEFF. (for Cy3: )
150000 (for Cy3: )
150000 (for Cy3: )
150000 (for Cy3: )
150000 (for Cy3: )
150000
CALCULATION
FOI MOLECULES
DYE / kb MOLECULES
DYE / kb MOLECULES
DYE / kb MOLECULES
DYE / kb
Parameter CY3 ALEXA546 ALEXA555 DYE550
ABS.COEFF. 150000 ------ 150000 ------
CORRECTION A
550
:
F
260
0.04 0.0 0.04 0.0
CORRECTION A
550
:
F
280
0.05 0.0 0.04 0.0
Parameter CY5 ALEXA647 DYE650
ABS.COEFF. 250000 239000 ------
CORRECTION A
650
: F
260
0.0 0.0 0.0
CORRECTION A
650
: F
280
0.05 0.03 0.0
EN
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perating manual