Eppendorf BioPhotometer plus - Manual (Page 13)

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4 Installation
BioPhotometer plus — Operating manual
4 Installation
4.1 Preparing installation
 Keep the transport carton and the packing material for subsequent safe transport or storage.
 Check the completeness of delivery based on the details of the scope of delivery (see
Delivery package on page 8).
 Check all parts for any transport damage.
4.2 Selecting location
Select the location for the BioPhotometer plus in accordance with the following criteria:
• 2 power sockets with ground conductor for the BioPhotometer plus and the printer.
• Solid laboratory bench with horizontal work surface
Space requirement of the device: 40 cm (with printer: 65 cm) width, 50 cm depth.
• Temperature: 15 to 35 °C. Avoid direct sunlight.
• Humidity: 25 to 75 % relative humidity.
• Atmospheric pressure: 70 to 106 kPa.
4.3 Connect device to the main power supply
1. Place the BioPhotometer plus onto a suitable work surface.
2. Ensure that the mains voltage and frequency match the details for the range of mains
voltages and frequencies on the device nameplate.
3. Connect the device to the power supply and switch it on from the mains power switch 8 (Fig. 1
on p. 8).
4. Remove the protective film from the device display.
4.4 Cuvettes
You can insert standard rectangular glass or plastic cuvettes into the cuvette shaft (outside
diameter 12.5 mm x 12.5 mm). The optical path height must be 8.5 mm above the cuvette base
and the total cuvette height must be at least 36 mm. The light beam in the cuvette is 1.0 mm wide
and 1.5 mm high.
The cuvettes must be optically transparent for the respective measuring wavelength. For
measurements in the UV range Eppendorf provides a plastic cuvette called UVette which is
transparent from wavelengths above 220 nm and therefore also suitable for the measurement of
nucleic acids.
Abb. 2: Overview of different cuvette types
Fig. 2: Overview of different cuvette types
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