Eppendorf BioPhotometer plus - Manual (Page 37)

37
8 Troubleshooting
BioPhotometer plus — Operating manual
8.2 Error messages
Device displays with error messages can be exited using Enter.
If certain parameters are programmed incompletely or incorrectly the icon for the parameter key
is shown in the display. In these cases press the parameter/dilution key instead. This brings you
directly to the parameter list to correct the error.
Error text Explanation / cause Remedy
(Parameter) PLEASE
PROGRAM
e.g.: FACTOR PLEASE
PROGRAM
Method parameter has not been programmed
correctly or is incomplete.  Check and correct parameter (see
Parameter on page 26).
MEASURE BLANK FIRST No blank has been stored for the method
opened.  Measure blank.
MEASURE STANDARD FIRST No calibration has been stored for the method
opened.
 Measure standards to save a valid
calibration.
NO STD METHOD The key standard has been pressed although
no calibration evaluation has been
programmed for the method.  Program evaluation with standards
in the measuring parameters or
measure the method again without
standards.
OUTSIDE OF CALIBRATION Only for non-linear calibration evaluation:
The measured sample absorbance is outside
the absorbance range of the standard.  Only measure samples which are
within the calibration range. For
too highly concentrated samples:
dilute samples.
MEASURED VALUES NOT
MONOTONIC For multi-point calibration:
The absorbance values of the standard do not
result in a monotonic increasing or decreasing
order.  Check standards and measure
again in the correct order
(increasing concentration).
CALIBRATION CURVE IS NOT
MONOTONIC The calibration curve is not monotonic
increasing or decreasing or does have a
reversal point.  Check standards, if necessary
prepare again and re-measure.
PROGRAM STANDARDS
INCREASING For multi-point calibration the target
concentration of the standards has not been
programmed in increasing order.  Program target concentration of
the standards in increasing order.
CV IS GREATER THAN 10% When calculating the calibration the "CV" is
greater than 10%.  Check standards, if necessary
prepare again and re-measure.
CALIBRATION NOT OK! The measured standard absorbances do not
result in a valid calibration evaluation.  Check standards and measure
again.
MEASURED VALUES NOT
PLAUSIBLE Value cannot be displayed; potential causes
when measuring with standards:
• Standard for single point calibration has the
absorbance "0"
• Calculated factor for single point calibration
is too great to be displayed.
• Polynome for multi-point calibration cannot
be displayed with the measured
absorbance values.
 For measurements with standards:
check measured absorbances; if
necessary, prepare standards
again and re-measure.
INVALID INPUT When entering a sample number:
The number "0" was entered  Enter sample number between 1
and 999.
MEASURING MODULE FAULT
1 (or 2 or 3) Technical error messages.  Contact Eppendorf Service.
EN
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