Eppendorf BioPhotometer plus - Manual (Page 35)

35
7 Maintenance
BioPhotometer plus — Operating manual
7.5.1 Test procedure
1. Open the function PHOTOMETER TEST / NEW MEASUREMENT.
2. Select the test filter and confirm the selection with "Enter".
3. "SAMPLE 260" or "SAMPLE 280" for measuring the accuracy of the wavelength for 260 or
280 nm.
4. "SAMPLE A1", "SAMPLE A2" or "SAMPLE A3" for measuring the photometric accuracy at
230, 260, 280, 340, 405, 490, 550, 595 and 650 nm.
5. Follow the instructions on the device display for measuring the blank ("A0") and the test filter.
The device carries out 10 measurements each and then displays the mean value and the CV
value for the measured absorbance at the respective wavelengths.
6. Press "Enter" in accordance with the instructions on the device display to have the values
displayed and then printed - provided a printer is connected.
7. Compare the mean values and the CV values to the permitted ranges in the table supplied.
If the measured values do not match the permitted value range please contact Eppendorf
Service. The filter should be recalibrated by Eppendorf after 2 years.
Hint!
• Carry out test at approx. 20°C.
• Remove filter only briefly from the filter box and protect against contamination or damage to
the filter surface.
• Protect filter against dust, heat, liquids and aggressive vapors.
• Always insert the filter with the sticker containing the filter designation facing the user
(towards the recipient).
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perating manual