5 photometer, 6 other technical parameters, Photometer – Eppendorf BioPhotometer plus User Manual
Page 41: Other technical parameters, 10 technical data, Operating manual
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10 Technical data
BioPhotometer plus — Operating manual
10.5 Photometer
10.6 Other technical parameters
Measuring principle
Absorption single beam photometer with reference
beam and several fixed wavelenghts
Light source
Xenon flash light
Monochromator
Holographic concave grating
Beam receiver
Silicon photo diodes
Wavelengths
230, 260, 280, 340, 405, 490, 550, 595, 650 nm
Wavelength selection
Dependent on method, controlled by program
Spectral bandwidth
5 nm at 230 to 340 nm
7 nm at 405 to 650 nm
Systematic wavelength error
± 1 nm at 230 to 280 nm
± 2 nm at 340 to 650 nm
Photometric measuring range
Quartz glass cuvette:
•
A = 0 to 3, except:
•
A = 0 to 2 at 340 nm
•
Only for dye methods:
A = 0 to 2 at 550/650 nm
UVette (Eppendorf):
•
A = 0 to 2.5 at 230 nm
•
A = 0 to 2.6 at 260 nm
•
A = 0 to 2.8 at 280 nm
•
Other values see quartz glass cuvette
Reading accuracy
ΔA = 0.001
Random photometric error
0.002 at A = 0
0.005 at A = 1
Systematic photometric error
± 1% at A = 1
Stray light component
< 0.05 %
Cuvette material
For DNA, RNA, Oligo, Protein UV, Assay 340:
Quartz glass or UV transparent plastic
(UVette by Eppendorf)
For OD600, Bradford, Lowry, BCA, Assay 405,
Assay 490:
Glass or plastic
Cuvette shaft
12.5 mm x 12,5 mm, not temperature-controlled
Overall cuvette height
Min. 36 mm
Height of the light beam in the cuvette
8.5mm
Light beam in the cuvette
Width: 1 mm
Height: 1.5 mm
Keyboard
19 foil keys
Display
Illuminated graphic display, 33 mm x 60 mm
Operator guidance language
English, French, German
Result output
Via display and printer:
Absorbance, concentration, ratio, FOI
Standards and regulations
According to VDE, CE, IEC 1010-1
EN
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perating manual