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5 photometer, 6 other technical parameters, Photometer – Eppendorf BioPhotometer plus User Manual

Page 41: Other technical parameters, 10 technical data, Operating manual

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41

10 Technical data

BioPhotometer plus — Operating manual

10.5 Photometer

10.6 Other technical parameters

Measuring principle

Absorption single beam photometer with reference
beam and several fixed wavelenghts

Light source

Xenon flash light

Monochromator

Holographic concave grating

Beam receiver

Silicon photo diodes

Wavelengths

230, 260, 280, 340, 405, 490, 550, 595, 650 nm

Wavelength selection

Dependent on method, controlled by program

Spectral bandwidth

5 nm at 230 to 340 nm
7 nm at 405 to 650 nm

Systematic wavelength error

± 1 nm at 230 to 280 nm
± 2 nm at 340 to 650 nm

Photometric measuring range

Quartz glass cuvette:

A = 0 to 3, except:

A = 0 to 2 at 340 nm

Only for dye methods: 
A = 0 to 2 at 550/650 nm

UVette (Eppendorf):

A = 0 to 2.5 at 230 nm

A = 0 to 2.6 at 260 nm

A = 0 to 2.8 at 280 nm

Other values see quartz glass cuvette

Reading accuracy

ΔA = 0.001

Random photometric error

 0.002 at A = 0

 0.005 at A = 1

Systematic photometric error

± 1% at A = 1

Stray light component

< 0.05 %

Cuvette material

For DNA, RNA, Oligo, Protein UV, Assay 340:
Quartz glass or UV transparent plastic 
(UVette by Eppendorf)
For OD600, Bradford, Lowry, BCA, Assay 405,
Assay 490:
Glass or plastic

Cuvette shaft

12.5 mm x 12,5 mm, not temperature-controlled

Overall cuvette height

Min. 36 mm

Height of the light beam in the cuvette

8.5mm

Light beam in the cuvette

Width: 1 mm
Height: 1.5 mm

Keyboard

19 foil keys

Display

Illuminated graphic display, 33 mm x 60 mm

Operator guidance language

English, French, German

Result output

Via display and printer:
Absorbance, concentration, ratio, FOI

Standards and regulations

According to VDE, CE, IEC 1010-1

EN

O

perating manual