Eppendorf BioPhotometer plus - Manual (Page 27)

27
6 Parameter and functions
BioPhotometer plus — Operating manual
REGRESSION Selection:
Linear and non-linear
regression Evaluation procedure of the multi-point calibration.
• Linear regression is possible for:
2 to 10 standards
• Non-linear regression is possible for:
(single measurement:) 5 to 10 standards
(double or triple measurement:) 4 to 10 standards.
STD 1 to STD 10 Numerical input (numbers and
decimal point, 5 digits) Nominal concentration for each standard. Unit as entered in
the parameter display 1.
The number of digits after the decimal point in the nominal
concentration of the first standard determines the number of
digits after the decimal point in the result. The
concentrations must be entered in ascending order.
CORRECTION Selection:
• NO CORRECTION
• CORRECTION A
340
• CORRECTION A
550 (or 650)
CORRECTION A
340
Can be used for the partial correction of the effect of minor
turbidity in the measuring solution: The absorbance
measured at 340 nm is subtracted from the absorbance
results at 230, 260 and 280 nm prior to subsequent
evaluation.
CORRECTION A
550 (or 650)
Can be used for the "Dye methods" to correct the effect of
the dye on the measuring absorbances of the biomolecule
(nucleic acid or protein): The absorbance of the dye
measured at 550 (or 650) nm is multiplied with a correction
factor for 260 or 280 nm. The result is subtracted from the
absorbances of the biomolecule at 260 or 280 nm prior to
subsequent evaluation for the biomolecule (see Dye-labeled
biomolecules ("dye methods") on page 22). The correction
factors are entered further down in the parameter list after
selecting the dye.
CY3 (or Cy5)
ALEXA ..... Selection For "Dye methods": selection of the dye (see Dye-labeled
biomolecules ("dye methods") on page 22).
ABS.COEFF. Numerical input (6 digits) For "Dye methods": input of the absorbance coefficient for
the selected dye in the unit "cm
-1 ·M -1 ".
CORRECTION 
A
550 (650)
: F
260
CORRECTION 
A
550 (650)
: F
260
Numerical input (5 digits) Only if in "Dye methods" the parameter 
CORRECTION A
550 (or 650)
has been enabled:
Entry of the correction factors for taking into account the
effect of the dye on the measurement absorbances of the
biomolecule (see description of the parameter
CORRECTION A
550 (or 650)
above).
CALCULATION FOI Selection:
• MOLECULE DYE / kb
• pmole/μg DNA (or RNA)
For "Dye methods": selection of the procedure for calculating
the frequency of incorporation (see Dye-labeled
biomolecules ("dye methods") on page 22).
Parameter Entry Explanation
EN
O
perating manual