Eppendorf BioPhotometer plus - Manual (Page 21)

21
5 Operation
BioPhotometer plus — Operating manual
5.6 Methods with evaluation via standards
For the following methods you can define an evaluation via calibration (measuring of standards)
in the parameters:
• PROTEIN 280nm (here only calibration with one standard is possible)
• BRADFORD, BRADFORD micro, BCA, BCA micro, LOWRY, LOWRY micro
• Methods via the keys "assays 340 / 405 / 490"
You can program up to 10 standards each in single up to triple measurement. For evaluation with
several standards you can choose between the procedures "Linear regression" (for calibration
lines) and "Non-linear regression" (for calibration curves). Dependent on the number of
programmed standards the following applies:
Measuring procedure
Standard measurements remain stored for an unlimited period of time after a valid calibration
until they are overwritten with a new calibration. Exception: If method parameters are modified
the calibration is deleted.
After opening a method (in the example the method "BRADFORD") you see the following device
display:
After saving the calibration you can continue with a sample measurement.
In the function list (see Functions on page 31) you can view and print the stored calibration data.
Evaluation procedure Number of standards
Calculation of a factor 1
Linear regression 2 to 10 standards
Non-linear regression for single measurement: 5 to 10 standards
for double or triple measurement: 4 to 10 standards
Hint!
The values marked "XXX" depend on the standard concentrations which you have programmed
in the method parameters.
Because a calibration has already been stored in this
example, the measuring keys provided include "sample" in
addition to "standard" and "blank".
Carry out a blank measurement.
Measure the first standard (in this example only as single
measurement).
Measure the other standards as prompted in the bottom
area of the device display.
After completing all standard measurements the calibration
has been evaluated and stored.
When measuring more than 2 standard samples a CV
value is displayed for the calculated regression. If the
calculated CV value exceeds 10% you are prompted for
approval before saving. Sample measurements are
evaluated using the last valid calibration.
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