Eppendorf BioPhotometer plus - Manual (Page 17)

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5 Operation
BioPhotometer plus — Operating manual
5.3 Summary of the measuring procedure
This section contains a summary of the key steps of a measuring procedure.
5.3.1 Prepare measurement
1. Switch on the device and, if necessary, the printer also.
The device is immediately ready for measuring after being switched on.
2. Have the cuvettes for the measurements available. When selecting the cuvettes observe the
respective instructions (see Cuvettes on page 13).
3. Provide the measuring solutions for measuring the blanks and, if necessary, the standards
and samples.
Measuring solutions for standards and samples with lower absorbances than 0.02 to 0.03 A
(this range corresponds e.g. to a dsDNA concentration of 1.0 to 1.5 μg/ml) should not be
used. Whilst the detection level of the photometer is well below these values, the effect of
interferences from the measuring solutions (particles, bubbles, turbidity) on the reliability of
the results is very high for these low absorbances.
4. Slide the cover of the cuvette shaft back to open the cuvette shaft.
5.3.2 Select the method
1. Using a key select the method group.
In the device display you see a list of methods provided in the selected group.
2. Using the cursor keys select the desired method.
3. Open the selected method using the Enter key.
The startup screen of the method is displayed:
Hint!
Before opening the method you should check the parameters of the desired method and correct
them, if necessary (see Parameter on page 26).
• Top: method name and programmed optical path
length of the cuvette.
• Center: programmed evaluation (e.g. factor or
information about the evaluation with standards).
• Bottom: keys for the next measurement. The keys
are not shown if there is insufficient space to display
them, but they can be made visible using the Enter
key in this case.
Hint!
The displayed factor defined in the method parameters always relates to an optical path length of
10 mm. However, for calculating the result the device automatically takes into account the optical
path length defined in the method parameters. Therefore, you do not need to modify the factor in
the method parameters for other optical path lengths.
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