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5 troubleshooting, 1 potential errors, 5 troubleshooting 5 – Eppendorf FemtoJet express User Manual

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Pressure loss due to capillary not fitted with capillary holder connected.
Solution: Connect the capillary or select Function 0,

Change capillary via the

key and variable regulator p

i

.

Capillary is blocked.
Solution: Press the

key for long enough.

Change capillary.
Purify injection fluid (e.g. by means of centrifugation).

Valve rattles
Solution: Connect tube to filled capillary.

Pressure control is designed for the tube connected.

No communication with the micromanipulator.
Solution: Check cables and connections.

System pressure ps (or external pressure) sinks to
less than 4,000 hPa (58 PSI). This is represented by
the instruction avove the entry Supply pressure in the
display.

If an injection or cleaning is activated in this status,
this will trigger Warning 1 (Chapter 5.2).

5.1 Potential errors

AUTO

n= 0

pi=

150 hPa

Apply supply pressure
Supply pressure

3999

pi[hPa]

ti[s]

pc[hPa]

150

0.2

50

Troubleshooting

5 Troubleshooting

5

05_Fehlersuche_en.fm Seite 78 Freitag, 29. Juni 2007 2:50 14