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4 clean function, 5 manual or automatic injection, 6 constant pressure / constant working pressure – Eppendorf FemtoJet express User Manual

Page 23: 3 mode of operation, Mode of operation

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3.4.4 Clean function
The Clean function is used to flush out blocked capillaries. The maximum available pressure is
applied to the outlet with the

key. It is applied for as long as the

key is pressed.

The maximum pressure of the FemtoJet

®

express is in the range of 7,000 hPa (102 psi) within

the range of the displayed system pressure. The value cannot be changed.

The pressure curve up to maximum pressure appears in the display in the form of a bar chart.
By pressing the

key briefly, a lower pressure can also be obtained.

3.4.5 Manual or automatic injection
In the Manu operating mode, injection is performed for as long as the

key or hand / foot

control is held down. The duration (t

i

) of injection is measured and shown in the display. In the

case of combination with an Eppendorf Micromanipulator, the injection movement is triggered.

In the Auto operating mode, the built-in timer facilitates a reproducible injection time.
The injection runs automatically for the duration of set time t

i

. It is triggered by the

key or

the hand / foot control being pressed briefly. During injection, the Inject key is
inactive, i.e. pressing it again has no effect. In combination with an Eppendorf Micromanipula-
tor, the automatic injection procedure can also be triggered from the operating panel of the
micromanipulator.

3.4.6 Constant pressure / constant working pressure
Using the Constant Pressure function, it is possible to select a higher pressure permanently if
required. This function is helpful when special injection techniques require either a high pressure
up to a maximum of 6,000 hPa (87 psi) or a permanent flow of injection solution
(see Section 3.7.5). Control accuracy drops in the pressure range from 2,000 – 6,000 hPa
(29 – 87 psi).

3 Mode of operation

Mode of operation

3

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