Eppendorf FemtoJet express - Manual (Page 20)

68
Other accessories
Grip head 0,
incl. 2 sets of O-rings and sealing washers.
Using grip head 0 supplied, capillaries with an outer diameter of 1.0 mm
to 1.1 mm can be used. In the case of outer diameters which differ from
these measurements, the relevant grip heads can be ordered as
non-standard accessories for the universal capillary holder
(see Section 7, Ordering information). The transparent grip head allows
the user to visually monitor the position of the capillary.
The following pressures can be used:
The usual values for the permanent compensation pressure P
c
are between 30 – 50 hPa
for Femtotips
®
and similar capillaries. The FemtoJet
®
express shows good, constant values
within this range Immediately after the device is switched on and maintains the balance bet-
ween undesired absorption of medium on the one hand and excessive loss of the liquid to be
injected on the other hand.
Quite low P
c
values are sometimes advisable for special applications (e.g. capillaries with an
interior diameter of 5 µm and greater)! In order to be able to also work precisely and efficiently
here, the FemtoJet
® express should be switched on app. 60 to 90 minutes before beginning the
experiment to guarantee the correct thermal settings. A pressure setting below 5 hPa is not
possible. You may merely also set 0 hPa.
Name Pressure range Comments
Compensation
pressure p
c
0; 5 – 6,000 hPa
0.00; 0.07 – 87.0 psi Or up to 500 hPa less than the
system pressure p
s
Injection pressure p
i
0; 5 – 6,000 hPa
0.00; 0.07 – 87.0 psi Or up to 500 hPa less than the
system pressure p
s
Clean pressure maximum
up to 7,000 hPa
maximum up to 102 psi
Constant working
pressure p
w
0; 5 – 6,000 hPa
0.00; 0.07 – 87.0 psi Or up to 500 hPa less than the
system pressure p
s
Select Function 4 via the
key.
System pressure p
s
[supply pressure]
maximum
up to 7,000 hPa
maximum up to 102 psi This is used directly for
.
3.4 Information on working practices
Mode of operation
3 Mode of operation
3
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