Eppendorf FemtoJet express - Manual (Page 15)

63
3.1.2 Variable regulators
The variable regulators are set so that when they are turned slowly, the values are changed by
1 hPa / 0.1 second per notch (0.01 – 0.02 psi).
If the regulators are turned faster, the values are altered in larger increments.
3.1.3 Keypad
Manual injection is performed for as long as the key is held down. Injection pressure p
i
is
used. In the second line, injection time t
i
is counted upwards from 0.0.
If the FemtoJet ® express is connected to the Eppendorf Micromanipulator InjectMan ® NI 2,
5171 or Inject Man ® the injection movement of the micromanipulator is also started via the
key. Both units then combine as a system to perform semi-automatic microinjection.
Alternatively, the INJECT key of the connected micromanipulator or the hand / foot control can
be used.
If synchronization is set to "limit" (SYNC = LIMIT) in the INJECT function of the micro-
manipulator, semi-automatic injection can only be triggered via the micromanipulator
.
The following settings are used as a basis for initial
injection experiments using Femtotip
® / Femtotip ® II:
Display:
p
i
left-hand variable regulator: 150 hPa
t
i
central variable regulator: 0.5 s
p
c
right-hand variable regulator: 50 hPa
Variable regulators:
Inject
Performs automatic or manual injection. Automatic
injection is controlled via the injection time t
i
, and the
injection pressure p
i
is used. In the second line, injection
time t
i
is counted down from the nominal value.
AUTO n= 0
pi= 150 hPa
pi[hPa] ti[s] pc[hPa]
150 0.5 50
AUTO - INJECT n= 0
0.4 s
Supply pressure 5261
pi[hPa] ti[s] pc[hPa]
150 0.6 50
3 Mode of operation
Mode of operation
3
03_Bedienung_en.fm Seite 63 Freitag, 29. Juni 2007 2:49 14