Eppendorf FemtoJet express - Manual (Page 14)

62
Before operating the device for the first time, users should read this section carefully. This
section describes operation of the device only. Handling of the injection capillaries is described
in Section 3.3.
3.1.1 Setting the injection parameters
The injection parameters have to be adapted to the injection capillaries used and the cell type
and liquid which are to be injected.
The following injection parameters can be set using the variable regulators:
Injection pressure p
i
0; 5 – 6,000 hPa (0.00; 0.07 – 87.0 psi)
Injection time t
i
0.1 – 99.9 s
Compensation pressure p
c
0; 5 – 6,000 hPa (0,00; 0.07 – 87.0 psi)
The settings are in increments of 1 hPa/0.1 s. The display can show hPa or psi, as required.
The setting and internal pressure conversion are always performed in hPa. Rounding for the
display in psi can result in increments of 0.01 to 0.02 psi.
The display of pressure in psi varies according to the size of the value (e.g.: 9.99 may also
appear in the display as 10.0).
Note:
An adjustment of p
i
or p
c
above the internally adjacent system pressure p
s
doesn't make sense.
The values for p
i
, p
c
should lie at least 500 hPa (7.2 PSI) below the system pressure in order to
ensure secure valve regulation.
Slight deviations between the nominal value (specified value) and displayed compensation
pressure p
c
(actual value) may occur for technical reasons. The relevant value is the actual value
measured.
Display
The display shows the following:
1st line:
injection mode AUTO or MANU,
counter n for number of injections performed
2nd line (bold):
The most-recently set parameter or relevant parameter
Middle line:
In the device, adjacent maximum system pressure p
s
.
Bottom lines:
Parameters which affect injection
Compensation pressure p
c
which is applied permanently in
the normal mode is displayed as an actual measured value.
The arrangement of the three parameters shown, p
i
, t
i
, p
c
,
corresponds to the arrangement of the variable regulators.
3.1 Display, variable regulators and keypad
AUTO n= 0
pi= 150 hPa
Supply pressure 5261
pi[hPa] ti[s] pc[hPa]
150 0.2 50
AUTO n= 0
pi= 2.15 PSI
Supply pressure 76.3
pi[PSI] ti[s] pc[PSI]
2.15 0.2 0.72
Mode of operation
3 Mode of operation
3
03_Bedienung_en.fm Seite 62 Freitag, 29. Juni 2007 2:49 14