Eppendorf FemtoJet express - Manual (Page 19)

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Universal capillary holder
The universal capillary holder is used to locate the injection capillaries. Prefabricated Eppendorf
Femtotips
® / Femtotips ® II (6) are screwed into the universal capillary holder using the adapter
(5). To do so, the adapter for Femtotips ® (5) is first screwed into the front rotatable knurled
screw (4) and then the Femtotip ® / Femtotip ® II is attached. If self-pulled capillaries are to be
attached, the grip head 0 (7) needs to be screwed loosely into the front rotatable knurled screw
(4). The self-pulled capillary is pushed into the front opening of the grip head through the two
O-rings and fixed in position by tightening the grip head.
Universal capillary holder
1 Tube connection
2 Rear knurled screw
3 Tension piece
4 Front knurled screw, rotatable
5 Adapter for Femtotips
®
(microcapillaries for microinjection)
6 Femtotip
®
/ Femtotip
®
II
7 Grip head 0, plastic, with two O-rings and sealing washers
Connecting the universal capillary holder
The two-meter-long injection tube is connected to the switched on FemtoJet
® express (1) via
the bayonet joint (2). The pressure tube (3) is screwed to the rear knurled screw (4) of the
universal capillary holder. Femtotips
® / Femtotips ® II are fitted at the front end of the universal
capillary holder (5) or independently-manufactured capillaries are clamped with the aid of the
appropriate grip head.
3.3 Connecting the capillary holder
1 2 3 4 5 6
7
1 2 3 4 5
3 Mode of operation
Mode of operation
3
03_Bedienung_en.fm Seite 67 Freitag, 29. Juni 2007 2:49 14